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Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis
Hexahistidines are very common tags used in the affinity chromatography purification of recombinant proteins. Although these tags are solely applied for their metal-binding properties, we found that they are also able to perform ester hydrolysis when attached to a protein. For instance, green fluore...
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| Publié dans: | Sci Rep |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group UK
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5676709/ https://ncbi.nlm.nih.gov/pubmed/29116178 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-15310-y |
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