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A protocol for gene expression analysis of chondrocytes from bovine osteochondral plugs used for biotribological applications

RNA isolation from human or animal cartilage tissue is necessary when performing mechanical or biotribological applications. Despite no influence on the cells and no alterations in gene expression patterns, enzymatic digestion of tissues should be avoided as it’s known that the expression of collage...

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Détails bibliographiques
Publié dans:MethodsX
Auteurs principaux: Bauer, Christoph, Niculescu-Morzsa, Eugenia, Nehrer, Stefan
Format: Artigo
Langue:Inglês
Publié: Elsevier 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5671390/
https://ncbi.nlm.nih.gov/pubmed/29124019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2017.10.005
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