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X-ray Radiation Induces Deprotonation of the Bilin Chromophore in Crystalline D. radiodurans Phytochrome

We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromop...

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Bibliographic Details
Published in:J Am Chem Soc
Main Authors: Li, Feifei, Burgie, E. Sethe, Yu, Tao, Héroux, Annie, Schatz, George C., Vierstra, Richard D., Orville, Allen M.
Format: Artigo
Language:Inglês
Published: 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5660918/
https://ncbi.nlm.nih.gov/pubmed/25650486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja510923m
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