In Vitro Seamless Stack Enzymatic Assembly of DNA Molecules Based on a Strategy Involving Splicing of Restriction Sites

The standard binary enzymatic assembly, which operates by inserting one DNA fragment into a plasmid, has a higher assembly success rate than the polynary enzymatic assembly, which inserts two or more fragments into the plasmid. However, it often leaves a nucleotide scar at the junction site. When a...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Yu, Dong, Tan, Yanning, Sun, Zhizhong, Sun, Xuewu, Sheng, Xiabing, Zhou, Tianshun, Liu, Ling, Mo, Yi, Jiang, Beibei, Ouyang, Ning, Yin, Xiaolin, Duan, Meijuan, Yuan, Dingyang
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5660187/
https://ncbi.nlm.nih.gov/pubmed/29079784
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-14496-5
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