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Detection of Wnt5 in Media Conditioned by Mouse Embryonic Fibroblast
This protocol describes the procedure of visualizing secreted Wnt5 protein in serum free media via western blotting. This procedure can also be used to visualize other secreted proteins larger than 10,000 daltons. The work presented in this paper visualizes Wnt5 secreted by mouse embryonic fibroblas...
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| 出版年: | Bio Protoc |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2016
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5659620/ https://ncbi.nlm.nih.gov/pubmed/29082284 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1971 |
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