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Suppression of Red Blood Cell Autofluorescence for Immunocytochemistry on Fixed Embryonic Mouse Tissue

Autofluorescence is a problem that interferes with immunofluorescent staining and complicates data analysis. Throughout the mouse embryo, red blood cells naturally fluoresce across multiple wavelengths, spanning the emission and excitation spectra of many commonly used fluorescent reporters, includi...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Curr Protoc Neurosci
Main Authors: Whittington, Niteace C., Wray, Susan
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5657453/
https://ncbi.nlm.nih.gov/pubmed/29058770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpns.35
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