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New reverse genetics and transfection methods to rescue arboviruses in mosquito cells

Reverse genetics is a critical tool to decrypt the biological properties of arboviruses. However, whilst reverse genetics methods have been usually applied to vertebrate cells, their use in insect cells remains uncommon due to the conjunction of laborious molecular biology techniques and of specific...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Atieh, Thérèse, Nougairède, Antoine, Klitting, Raphaëlle, Aubry, Fabien, Failloux, Anna-Bella, de Lamballerie, Xavier, Priet, Stéphane
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5656662/
https://ncbi.nlm.nih.gov/pubmed/29070887
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-14522-6
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