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Express FRET Labeling and Analysis of Phagocytic Clearance
Lysosomal DNase II in phagocytic digestion produces DNA ends with 3′PO(4)/5′OH, which differ from those created in apoptotic DNA fragmentation, and can be used to label phagocytic clearance of cell death. Here, we describe the use of these specific DNA ends as selective markers of phagocytic reactio...
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Publicado no: | Methods Mol Biol |
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Autor principal: | |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5654643/ https://ncbi.nlm.nih.gov/pubmed/28710749 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7187-9_1 |
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