Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity

OBJECTIVES: To evaluate the effects of the limiting dilution method and plating density in rat nucleus pulposus mesenchymal stem/progenitor cells (NPMSCs). MATERIALS AND METHODS: Nucleus pulposus tissues were isolated from 12-week-old male Sprague-Dawley rats and NPMSCs were isolated using limiting...

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Publicado en:Biomed Res Int
Autores principales: Lin, Linghan, Jia, Zhiwei, Zhao, Yachao, Wu, Yaohong, Zhao, Xiyan, Li, Yong, Guo, Ziming, Chen, Jiahai, Cheng, Shi, Wang, Deli, Ruan, Dike
Formato: Artigo
Lenguaje:Inglês
Publicado: Hindawi 2017
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5651100/
https://ncbi.nlm.nih.gov/pubmed/29119116
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2017/9765843
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