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3D morphological analysis of the mouse cerebral vasculature: Comparison of in vivo and ex vivo methods

Ex vivo 2-photon fluorescence microscopy (2PFM) with optical clearing enables vascular imaging deep into tissue. However, optical clearing may also produce spherical aberrations if the objective lens is not index-matched to the clearing material, while the perfusion, clearing, and fixation procedure...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Steinman, Joe, Koletar, Margaret M., Stefanovic, Bojana, Sled, John G.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5650181/
https://ncbi.nlm.nih.gov/pubmed/29053753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0186676
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