Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations

We present speed out-of-phase imaging after optical modulation (OPIOM), which exploits reversible photoswitchable fluorophores as fluorescent labels and combines optimized periodic illumination with phase-sensitive detection to specifically retrieve the label signal. Speed OPIOM can extract the fluo...

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Veröffentlicht in:Nat Commun
Hauptverfasser: Quérard, Jérôme, Zhang, Ruikang, Kelemen, Zsolt, Plamont, Marie-Aude, Xie, Xiaojiang, Chouket, Raja, Roemgens, Insa, Korepina, Yulia, Albright, Samantha, Ipendey, Eliane, Volovitch, Michel, Sladitschek, Hanna L., Neveu, Pierre, Gissot, Lionel, Gautier, Arnaud, Faure, Jean-Denis, Croquette, Vincent, Le Saux, Thomas, Jullien, Ludovic
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5645393/
https://ncbi.nlm.nih.gov/pubmed/29042541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-00847-3
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