Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli

BACKGROUND: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system METHODS: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the ve...

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Dades bibliogràfiques
Publicat a:Rep Biochem Mol Biol
Autors principals: Khademi, Farzad, Yousefi-Avarvand, Arshid, Derakhshan, Mohammad, Meshkat, Zahra, Tafaghodi, Mohsen, Ghazvini, Kiarash, Aryan, Ehsan, Sankian, Mojtaba
Format: Artigo
Idioma:Inglês
Publicat: Varastegan Institute for Medical Sciences 2017
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Original Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5643456/
https://ncbi.nlm.nih.gov/pubmed/29090225
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