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Temporal calcium profiling of specific circadian neurons in freely moving flies

There are no general methods for reliably assessing the firing properties or even calcium profiles of specific neurons in freely moving flies. To this end, we adapted a GFP-based calcium reporter to luciferase that was expressed in small subsets of circadian neurons. This Tric-LUC reporter allowed a...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Guo, Fang, Chen, Xiao, Rosbash, Michael
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5642695/
https://ncbi.nlm.nih.gov/pubmed/28973886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1706608114
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