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Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters

Rat embryonic stem cells (ESCs) offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here, we used the CRISPR/Cas9 system to target genomi...

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Gepubliceerd in:Stem Cell Reports
Hoofdauteurs: Chen, Yaoyao, Spitzer, Sonia, Agathou, Sylvia, Karadottir, Ragnhildur Thora, Smith, Austin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2017
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5639479/
https://ncbi.nlm.nih.gov/pubmed/29020614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2017.09.005
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