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Development and Characterization of a Synthetic DNA, NUversa, to Be Used as a Standard in All Quantitative PCR Reactions for Molecular Pneumococcal Serotyping

BACKGROUND: Identification of Streptococcus pneumoniae (Spn) and its more than 90 serotypes is routinely conducted by culture and Quellung reactions. Quantitative (q)PCR reactions have been developed for molecular detection, including a pan-Spn lytA assay, and assays targeting 78 serotypes. Reaction...

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Detalhes bibliográficos
Publicado no:Open Forum Infect Dis
Main Authors: Sakai, Fuminori, Sonaty, Griffin, Klugman, Keith, Vidal, Jorge
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5630758/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/ofid/ofx163.1622
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