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Characterization of the Dihydroorotase from Methanococcus jannaschii
The gene that codes for the putative dihydroorotase in the hyperthermophilic archaeon Methanococcus jannaschii was subcloned in pET-21a and expressed in Escherichia coli. A purification protocol was devised. The purity of the protein was evaluated by SDS-PAGE and the protein was confirmed by sequenc...
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| Publicado no: | Protein J |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5624517/ https://ncbi.nlm.nih.gov/pubmed/28660315 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10930-017-9729-7 |
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