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First attempts to crystallize a non-homogeneous sample of thioredoxin from Litopenaeus vannamei: What to do when you have diffraction data of a protein that is not the target?

The importance of sample homogeneity and purity in protein crystallization is essential to obtain high-quality diffracting crystals. Here, in an attempt to determine the crystal structure of thioredoxin 1 from whiteleg shrimp Litopenaeus vannamei (LvTrx), we inadvertently crystallized the hexameric...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Rep
Main Authors: Campos-Acevedo, Adam A., Díaz-Vilchis, Adelaida, Sotelo-Mundo, Rogerio R., Rudiño-Piñera, Enrique
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614465/
https://ncbi.nlm.nih.gov/pubmed/28955968
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2016.09.011
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