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Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Transfer RNA (tRNA) is an essential part of the translational machinery in any organism. tRNAs bind and transfer amino acids to the translating ribosome. The relative levels of different tRNAs, and the ratio of aminoacylated tRNA to total tRNA, known as the charging level, are important factors in d...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Stenum, Thomas Søndergaard, Sørensen, Michael A., Svenningsen, Sine Lo
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614356/
https://ncbi.nlm.nih.gov/pubmed/28872118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56212
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