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A cost-effective protocol for the over-expression and purification of fully-functional and more stable Erwinia chrisanthemi ligand-gated ion channel

The Erwinia chrisanthemi ligand-gated ion channel, ELIC, is considered an excellent structural and functional surrogate for the whole pentameric ligand-gated ion channel family. Despite its simplicity, ELIC is structurally capable of undergoing ligand-dependent activation and a concomitant desensiti...

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Detalhes bibliográficos
Publicado no:Protein Expr Purif
Main Authors: Elberson, Benjamin W., Whisenant, Ty E., Cortes, D. Marien, Cuello, Luis G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5612499/
https://ncbi.nlm.nih.gov/pubmed/28279818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2017.03.006
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