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Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Cell-surface proteins mediate a wide array of functions. In many cases, their activity is regulated by endocytic processes that modulate their levels at the plasma membrane. Here, we present detailed protocols for 2 methods that facilitate the study of such processes, both of which are based on the...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Tham, Daniel Kai Long, Moukhles, Hakima
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608537/
https://ncbi.nlm.nih.gov/pubmed/28715371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55974
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