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Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N(6)-threonylcarbamoyladenosine TcdA Function
We demonstrate methods for the expression and purification of tRNA(UUU) in Escherichia coli and the analysis by gel retardation assays of the binding of tRNA(UUU) to TcdA, an N(6)-threonylcarbamoyladenosine (t(6)A) dehydratase, which cyclizes the threonylcarbamoyl side chain attached to A37 in the a...
Guardat en:
| Publicat a: | J Vis Exp |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
MyJove Corporation
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5608462/ https://ncbi.nlm.nih.gov/pubmed/28671653 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55638 |
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