Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

This work describes the whole-mount immunohistochemistry staining method in detail, using neurofilament protein antibody to label the innervation of the biliary tract in Suncus murinus (S. murinus ). First, the specimen was dissected from S. murinus and fixed in 4% paraformaldehyde (PFA). Second, an...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Ren, Ke, Dai, Yidan, Yi, Kai, Kinoshita, Masanobu, Itoh, Masahiro, Sakata, Ichiro, Sakai, Takafumi, Yi, Shuang-Qin
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2017
Subjects:
Physiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608420/
https://ncbi.nlm.nih.gov/pubmed/28654062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55483
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