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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

The dedifferentiation of hyaline chondrocytes into fibroblastic chondrocytes often accompanies monolayer expansion of chondrocytes in vitro. The global DNA methylation level of chondrocytes is considered to be a suitable biomarker for the loss of the chondrocyte phenotype. However, results based on...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Jia, Zhaofeng, Liang, Yujie, Ma, Bin, Xu, Xiao, Xiong, Jianyi, Duan, Li, Wang, Daping
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5607994/
https://ncbi.nlm.nih.gov/pubmed/28570531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55565
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