Measuring Protein Binding to F-actin by Co-sedimentation

Filamentous actin (F-actin) organization within cells is regulated by a large number of actin-binding proteins that control actin nucleation, growth, cross-linking and/or disassembly. This protocol describes a technique – the actin co-sedimentation, or pelleting, assay – to determine whether a prote...

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Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Heier, Jonathon A., Dickinson, Daniel J., Kwiatkowski, Adam V.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2017
Konular:
Biochemistry
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5607963/
https://ncbi.nlm.nih.gov/pubmed/28570520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55613
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