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Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies
A fusion protein SBP-Cap∆41, consisting of Cap∆41 (without 41 amino acids at the N-terminus) protein of porcine circovirus 2 (PCV2) and a streptavidin binding peptide (SBP), was constructed. This fusion protein binds to HRP-labeled streptavidin (HRP-SA) through high affinity between SBP and SA, form...
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Publicado no: | AMB Express |
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Main Authors: | , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Springer Berlin Heidelberg
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5603472/ https://ncbi.nlm.nih.gov/pubmed/28921455 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-017-0473-3 |
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