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Creating Knock-outs of Conserved Oligomeric Golgi complex subunits using CRISPR-mediated gene editing paired with a selection strategy based on glycosylation defects associated with impaired COG complex function

The Conserved Oligomeric Golgi (COG) complex is a key evolutionally conserved multisubunit protein machinery that regulates tethering and fusion of intra-Golgi transport vesicles. The Golgi apparatus specifically promotes sorting and complex glycosylation of glycoconjugates. Without proper glycosyla...

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Publicat a:Methods Mol Biol
Autors principals: Blackburn, Jessica Bailey, Lupashin, Vladimir V.
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5603234/
https://ncbi.nlm.nih.gov/pubmed/27632008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6463-5_12
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