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Metabolic engineering for recombinant major ampullate spidroin 2 (MaSp2) synthesis in Escherichia coli
In this research, metabolic engineering was employed to synthesize the artificial major ampullate spidroin 2 (MaSp2) in the engineered Escherichia coli. An iterative seamless splicing strategy was used to assemble the MaSp2 gene, which could reach 10000 base pairs, and more than 100 kDa protein was...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5595792/ https://ncbi.nlm.nih.gov/pubmed/28900203 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-11845-2 |
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