Development of a multiplex PCR assay for diarrheagenic Escherichia coli and Shigella spp. and its evaluation on colonies, culture broths, and stool

Detection of diarrheagenic E. coli (DEC) typically depends on identification of virulence genes from stool cultures, not on stool itself. We developed a multiplex PCR assay that detects key DEC virulence genes (stx1, stx2, eae, bfpA, ipaH, LT, STh, aaiC, aatA). The assay involved a multiplex PCR rea...

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izdano v:Diagn Microbiol Infect Dis
Main Authors: Taniuchi, Mami, Walters, Carol C., Gratz, Jean, Maro, Athanasia, Kumburu, Happiness, Serichantalergs, Oralak, Sethabutr, Orntipa, Bodhidatta, Ladaporn, Kibiki, Gibson, Toney, Denise M., Berkeley, Lynette, Nataro, James P., Houpt, Eric R.
Format: Artigo
Jezik:Inglês
Izdano: 2012
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5592968/
https://ncbi.nlm.nih.gov/pubmed/22541788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.diagmicrobio.2012.03.008
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