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HPLC-based quantification of bacterial housekeeping nucleotides and alarmone messengers ppGpp and pppGpp

Here we describe an HPLC-based method to quantify bacterial housekeeping nucleotides and the signaling messengers ppGpp and pppGpp. We have replicated and tested several previously reported HPLC-based approaches and assembled a method that can process 50 samples in three days, thus making kineticall...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Varik, Vallo, Oliveira, Sofia Raquel Alves, Hauryliuk, Vasili, Tenson, Tanel
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5591245/
https://ncbi.nlm.nih.gov/pubmed/28887466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-10988-6
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