A thiol probe for measuring unfolded protein load and proteostasis in cells

When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate. Here we report that the dye, tetraphenylethene maleimide (TPE-MI) can be used to measure cellular unfolded protein load. TPE-MI fluorescence is activated upon labelling free cysteine thiols, normally buried in the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nat Commun
Hauptverfasser: Chen, Moore Z., Moily, Nagaraj S., Bridgford, Jessica L., Wood, Rebecca J., Radwan, Mona, Smith, Trevor A., Song, Zhegang, Tang, Ben Zhong, Tilley, Leann, Xu, Xiaohong, Reid, Gavin E., Pouladi, Mahmoud A., Hong, Yuning, Hatters, Danny M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
Schlagworte:
Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5589734/
https://ncbi.nlm.nih.gov/pubmed/28883394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-00203-5
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge