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Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases

We have purified GST-fused recombinant mouse Dnmt3a and three isoforms of mouse Dnmt3b to near homogeneity. Dnmt3b3, an isoform of Dnmt3b, did not have DNA methylation activity. Dnmt3a, Dnmt3b1 or Dnmt3b2 showed similar activity toward poly(dG-dC)-poly(dG-dC) for measuring de novo methylation activi...

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Published in:Nucleic Acids Res
Main Authors: Aoki, Asako, Suetake, Isao, Miyagawa, Junichi, Fujio, Takayuki, Chijiwa, Takahito, Sasaki, Hiroyuki, Tajima, Shoji
Format: Artigo
Language:Inglês
Published: Oxford University Press 2001
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55888/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11522819/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.17.3506
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