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Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis

The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co(2+) and Mn(2+). Under these reaction conditions, the enzyme displayed K(m) and k(cat) value...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Sierra, Elwi Machado, Pereira, Mariana Rangel, Maester, Thaís Carvalho, Gomes-Pepe, Elisangela Soares, Mendoza, Elkin Rodas, Lemos, Eliana G. de Macedo
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5587760/
https://ncbi.nlm.nih.gov/pubmed/28878230
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-10932-8
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