Optimal conditions to use Pfu exo(–) DNA polymerase for highly efficient ligation-mediated polymerase chain reaction protocols

Ligation-Mediated Polymerase Chain Reaction (LMPCR) is the most sensitive sequencing technique available to map single-stranded DNA breaks at the nucleotide level of resolution using genomic DNA. LMPCR has been adapted to map DNA damage and reveal DNA–protein interactions inside living cells. Howeve...

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Bibliografske podrobnosti
Main Authors: Angers, Martin, Cloutier, Jean-François, Castonguay, André, Drouin, Régen
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2001
Teme:
NAR Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC55867/
https://ncbi.nlm.nih.gov/pubmed/11504891
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