Changing the target base specificity of the EcoRV DNA methyltransferase by rational de novo protein-design
The EcoRV DNA-(adenine-N(6))-methyltransferase (M.EcoRV) specifically modifies the first adenine residue within GATATC sequences. During catalysis, the enzyme flips its target base out of the DNA helix and binds it into a target base binding pocket which is formed in part by Lys16 and Tyr196. A cyto...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55820/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11470870/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.15.3137 |
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