Sensitive and selective quantification of free and total malondialdehyde in plasma using UHPLC-HRMS

Quantification of malondialdehyde (MDA) as a marker of lipid peroxidation is relevant for many research fields. We describe a new sensitive and selective method to measure free and total plasmatic MDA using derivatization with 2,4-dinitrophenylhydrazine (DNPH) and ultra-HPLC-high-resolution MS. Free...

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Bibliographic Details
Published in:J Lipid Res
Main Authors: Mendonça, Rute, Gning, Ophélie, Di Cesaré, Claudia, Lachat, Laurence, Bennett, Nigel C., Helfenstein, Fabrice, Glauser, Gaétan
Format: Artigo
Language:Inglês
Published: The American Society for Biochemistry and Molecular Biology 2017
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5580889/
https://ncbi.nlm.nih.gov/pubmed/28694297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D076661
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