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Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector
Development of CRISPR/Cas9 transient gene editing screening tools in plant biology has been hindered by difficulty of delivering high quantities of biologically active single guide RNAs (sgRNAs). Furthermore, it has been largely accepted that in vivo generated sgRNAs need to be devoid of extraneous...
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| Published in: | Plant Physiol |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society of Plant Biologists
2017
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5580747/ https://ncbi.nlm.nih.gov/pubmed/28663331 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.17.00411 |
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