Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor.

A nuclear extract prepared from HeLa cells has been used to study in vitro the transcription of the SV40 early promoter. The deletion of the enhancer results in a strong decrease of transcription, with spermidine and MgCl2 being critical variables in the transcription reactions. Furthermore a compet...

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Bibliographic Details
Published in:EMBO J
Main Authors: Wildeman, A G, Sassone-Corsi, P, Grundström, T, Zenke, M, Chambon, P
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 1984
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC557828/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6098465/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1984.tb02269.x
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