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Single-nucleotide resolution of RNAs up to 59 nucleotides by HPLC
Ion-pair, reverse-phase high performance liquid chromatography (HPLC) is a standard analytical platform for separating, purifying and analyzing RNAs. However, a single-nucleotide resolution by using HPLC is currently limited to RNAs shorter than 25 nucleotides (nt). Here we describe a method of sepa...
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| Veröffentlicht in: | Anal Biochem |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5577504/ https://ncbi.nlm.nih.gov/pubmed/23274387 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.12.011 |
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