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Synthetic donor and acceptor splice sites function in an RNA polymerase B (II) transcription unit.

We have synthesised a 32-bp oligonucleotide containing sequences conforming to the consensus sequences for donor and acceptor splice sites. The oligonucleotide has been inserted into an RNA polymerase B (II) transcription unit and the resulting recombinant used to study the splicing mechanism. Our f...

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Bibliographic Details
Published in:EMBO J
Main Authors: Rautmann, G, Matthes, H W, Gait, M J, Breathnach, R
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 1984
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC557637/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6489319/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1984.tb02085.x
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