An Assessment of Fixed and Native Chromatin Preparation Methods to Study Histone Post-Translational Modifications at a Whole Genome Scale in Skeletal Muscle Tissue

BACKGROUND: Genomic loci associated with histone marks are typically analyzed by immunoprecipitation of the chromatin followed by quantitative-PCR (ChIP-qPCR) or high throughput sequencing (ChIP-seq). Chromatin can be either cross-linked (X-ChIP) or used in the native state (N-ChIP). Cross-linking o...

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Detalhes bibliográficos
Publicado no:Biol Proced Online
Main Authors: David, Sarah-Anne, Piégu, Benoît, Hennequet-Antier, Christelle, Pannetier, Maëlle, Aguirre-Lavin, Tiphaine, Crochet, Sabine, Bordeau, Thierry, Couroussé, Nathalie, Brionne, Aurélien, Bigot, Yves, Collin, Anne, Coustham, Vincent
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5576305/
https://ncbi.nlm.nih.gov/pubmed/28855851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-017-0059-0
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