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Electroporation as a method for high-level nonviral gene transfer to the lung
To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline was instilled into the lungs of anesthetized mice. Plasmids expressed luciferase, or β-galactosidas...
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| 發表在: | Gene Ther |
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| Main Authors: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2003
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5576138/ https://ncbi.nlm.nih.gov/pubmed/12907953 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.gt.3302053 |
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