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Electroporation as a method for high-level nonviral gene transfer to the lung

To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline was instilled into the lungs of anesthetized mice. Plasmids expressed luciferase, or β-galactosidas...

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Detalhes bibliográficos
Publicado no:Gene Ther
Main Authors: Dean, DA, Machado-Aranda, D, Blair-Parks, K, Yeldandi, AV, Young, JL
Formato: Artigo
Idioma:Inglês
Publicado em: 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5576138/
https://ncbi.nlm.nih.gov/pubmed/12907953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.gt.3302053
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