Real-time detection of gene promoter activity: quantitation of toxin gene transcription

We have developed a new method for quantification of promoter activity in cell lines transfected with recombinant plasmids containing the reporter gene encoding chloramphenicol acetyl transferase (CAT) by real-time PCR. As the efficiency of transfection has a direct influence on the total mRNA produ...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Jeyaseelan, Kandiah, Ma, Donghui, Armugam, Arunmozhiarasi
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55757/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11410681/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.12.e58
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