Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer

We have developed a modified RNA interference (RNAi) method for generating gene knock-outs in Drosophila melanogaster. We used the sequence of the yellow (y) locus to construct an inverted repeat that will form a double-stranded hairpin structure (y-IR) that is under the control of the upstream acti...

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Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Piccin, Alberto, Salameh, Ahmad, Benna, Clara, Sandrelli, Federica, Mazzotta, Gabriella, Zordan, Mauro, Rosato, Ezio, Kyriacou, Charalambos P., Costa, Rodolfo
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2001
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55754/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11410678/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.12.e55
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