Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer

We have developed a modified RNA interference (RNAi) method for generating gene knock-outs in Drosophila melanogaster. We used the sequence of the yellow (y) locus to construct an inverted repeat that will form a double-stranded hairpin structure (y-IR) that is under the control of the upstream acti...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Piccin, Alberto, Salameh, Ahmad, Benna, Clara, Sandrelli, Federica, Mazzotta, Gabriella, Zordan, Mauro, Rosato, Ezio, Kyriacou, Charalambos P., Costa, Rodolfo
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2001
Gaiak:
NAR Methods Online
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC55754/
https://ncbi.nlm.nih.gov/pubmed/11410678
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