Fast free-of-acrylamide clearing tissue (FACT)—an optimized new protocol for rapid, high-resolution imaging of three-dimensional brain tissue

Fast Free-of-Acrylamide Clearing Tissue (FACT) is a new sodium dodecyl sulfate (SDS)-based clearing protocol for the chemical clearing and imaging of brain tissue containing transgenic or immunolabeled fluorescent proteins. In the present study, we have developed this new method and optimized multip...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Xu, Na, Tamadon, Amin, Liu, Yaan, Ma, Tong, Leak, Rehana K., Chen, Jun, Gao, Yanqin, Feng, Yi
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5575133/
https://ncbi.nlm.nih.gov/pubmed/28852046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-10204-5
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