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Kinetic modelling: an integrated approach to analyze enzyme activity assays

BACKGROUND: In general, enzyme activity is estimated from spectrophotometric data, by taking the slope of the linear part of the progress curve describing the rate of change in the substrate or product monitored. As long as the substrate concentrations are sufficiently high to saturate the enzyme an...

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Detalhes bibliográficos
Publicado no:Plant Methods
Main Authors: Boeckx, Jelena, Hertog, Maarten, Geeraerd, Annemie, Nicolai, Bart
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5574136/
https://ncbi.nlm.nih.gov/pubmed/28855956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-017-0218-y
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