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Automation of PRM-dependent D3-Leu tracer enrichment in HDL to study the metabolism of apoA-I, LCAT and other apolipoproteins

We developed an automated quantification workflow for PRM-enabled detection of D3-Leu labeled apoA-I in HDL isolated from humans. Subjects received a bolus injection of D3-Leu and blood was drawn at seven time points over three days. HDL was isolated and separated into six size fractions for subsequ...

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Publicado en:Proteomics
Autores principales: Lee, Lang Ho, Andraski, Allison B., Pieper, Brett, Higashi, Hideyuki, Sacks, Frank M., Aikawa, Masanori, Singh, Sasha A.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2017
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5572145/
https://ncbi.nlm.nih.gov/pubmed/27862954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201600085
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