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Neutron crystallography of photoactive yellow protein reveals unusual protonation state of Arg52 in the crystal

Because of its high pK(a), arginine (Arg) is believed to be protonated even in the hydrophobic environment of the protein interior. However, our neutron crystallographic structure of photoactive yellow protein, a light sensor, demonstrated that Arg52 adopts an electrically neutral form. We also show...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Yonezawa, Kento, Shimizu, Nobutaka, Kurihara, Kazuo, Yamazaki, Yoichi, Kamikubo, Hironari, Kataoka, Mikio
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5570954/
https://ncbi.nlm.nih.gov/pubmed/28839266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-09718-9
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