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Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy

We introduce a concept that enables parallelized three-dimensional imaging throughout large volumes with isotropic 300–350 nm resolution. By staggering high aspect ratio illumination beams laterally and axially within the depth of focus of a digitally scanned light-sheet fluorescence microscope (LSF...

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Pubblicato in:Sci Rep
Autori principali: Dean, Kevin M., Fiolka, Reto
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5570909/
https://ncbi.nlm.nih.gov/pubmed/28839150
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-08113-8
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