Characterization of the bacteriophage lambda excisionase (Xis) protein: the C-terminus is required for Xis-integrase cooperativity but not for DNA binding.
We have performed a mutational analysis of the xis gene of bacteriophage lambda. The Xis protein is 72 amino acids in length and required for excisive recombination. Twenty-six mutants of Xis were isolated that were impaired or deficient in lambda excision. Mutant proteins that contained amino acid...
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| Published in: | EMBO J |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Nature Publishing Group
1992
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC556840/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1396573/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1992.tb05465.x |
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