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Alanine-scanning Mutagenesis of Aspergillus γ-Tubulin Yields Diverse and Novel Phenotypes

We have created 41 clustered charged-to-alanine scanning mutations of the mipA, γ-tubulin, gene of Aspergillus nidulans and have created strains carrying these mutations by two-step gene replacement and by a new procedure, heterokaryon gene replacement. Most mutant alleles confer a wild-type phenoty...

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Bibliografiske detaljer
Main Authors: Jung, M. Katherine, Prigozhina, Natalie, Oakley, C. Elizabeth, Nogales, Eva, Oakley, Berl R.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Cell Biology 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC55667/
https://ncbi.nlm.nih.gov/pubmed/11452008
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